Expression of HOTAIR and MEG3 is negatively associated with H. pylori positivity in gastric cancer patients
A new research paper has been published in Genes & Cancer on February 10, 2022, titled: “Expression of HOTAIR and MEG3 is negatively associated with H. pylori positivity in gastric cancer patients.”
Chronic infection with Helicobacter pylori is one of the main causes of stomach disease cancer (GC). Besides, lncRNA plays an important role in cancer pathogenesis including GC.
In this study, researchers Farnaz Amini, Mohammad Khalaj-Kondori, Amin Moqadami and Ali Rajabi from the University of Tabriz aimed to investigate the expression of MEG3 and HOTAIR in gastric cancer tissues and evaluate the association of MEG3 and HOTAIR in gastric cancer tissues. them with H. pylori status.
“Here, we evaluated the expression of lncRNAs MEG3 and HOTAIR in GC and analyzed their association with H. pylori status and other pathological clinical features of the patient. Furthermore, potency. of their biomarkers were evaluated by Receiver Operating Characteristics (ROC) curve analysis.” mentioned researchers.
One hundred samples were taken. Total RNA was extracted, cDNA was synthesized and the expression of MEG3 and HOTAIR was assessed using qRT-PCR. The relationship between their expression and H. pylori infection and other clinical features was studied. Furthermore, the sensitivity and specificity of MEG3 and HOTAIR expression levels to distinguish tumor and non-tumor samples were evaluated by Receiver activity characteristic (ROC) curve analysis.
“We observed an up-regulation of HOTAIR but a down-regulation of MEG3 in tumors compared with non-tumor tissues. We also found a significant negative association between their expression levels. and H. pylori positive status.”
However, only the expression level of HOTAIR was significantly associated with tumor size and stage (P
“Expression of MEG3 and HOTAIR is negatively associated with H. pylori positivity. MEG3 is down-regulated but HOTAIR is up-regulated in GC tissues. Expression of HOTAIR is associated with size and stage segment of the tumor.Moreover, MEG3 and HOTAIR expression level could be considered as potential diagnostic biomarkers for GC,” the researchers concluded.
Farnaz Amini et al., Expression of HOTAIR and MEG3 is negatively associated with H. pylori positivity in gastric cancer patients, Genes & Cancer (2022). DOI: 10.18632/genesandCancer.219
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